DENARASE High Salt is an engineered version of the wild-type Serratia marcescens endonuclease, which is commonly used for DNA removal applications in bioprocessing. Compared to the wild-type DENARASE, a few amino acids were substituted to provide the enzyme with an improved salt tolerance without losing its specificity for nucleic acids.
- Designed for improved salt and pH tolerance: For highest process flexibility and efficiency
- Tailored to fit your process: Seamless integration into existing processes
- Compatible with DENARASE ELISA Kit: The one-for-all Serratia marcescens ELISA Kit
Like the wild-type S. marcescens endonuclease, DENARASE High Salt is capable of the non-specific cleavage of phosphodiester bonds between the nucleotide subunits of nucleic acids. The enzyme can hydrolyze all forms of DNA and RNA, such as single-stranded, double-stranded, linear, circular, or supercoiled.
The protein consists of two monomers with a calculated molecular weight of 27 kDa each. The calculated isoelectric point (pI) of DENARASE High Salt is at pH 7.83. Magnesium (Mg2+) is an essential co-factor and prerequisite for the nucleic acid clearance activity of the salt-tolerant endonuclease.
Similar to the wild-type DENARASE, the manufacturing process of DENARASE High Salt is based on recombinant expression in a Bacillus sp. production host. The gram-positive production strain is free of endotoxins. DENARASE High Salt is manufactured and supplied by c-LEcta GmbH.
DENARASE High Salt enables optimal and cost-effective removal of nucleic acids in biomanufacturing processes that benefit from higher salt concentrations. The enzyme is especially suitable for the use at bioprocessing-relevant 200-500 mM salt and pH 7.4-pH 8. It is designed to improve the efficiency of various applications:
Viral Vaccines
Viral Vectors for Cell and Gene Therapies
Viscosity Reduction in Lysates
Sample Preparation in Electrophoresis and Chromatography
Enzyme origin: Engineered from Serratia marcescens
Production host: Bacillus sp.
Temperature optimum: 37 °C
Magnesium optimum: 5 - 25 mM
Isoelectrical point: pH 7.83
Salt: 200 - 500 mM
Certifications: Produced under ISO 9001 standard, without the use of antibiotics, Triton X-100 and raw materials of animal origin or TSE/BSE risk.
Delivery information: DENARASE High Salt is shipped by default in insulated boxes with cold packs as a precautionary measure to protect against unexpectedly elevated temperatures during transport. Storage at -20 °C ± 5 °C is recommended upon receipt of the product.
Packaging: DENARASE High Salt is filled in non-pyrogenic, USP Class VI compliant vials. The product vials are shipped under qualified cooled conditions. Shipping temperature may differ from storage temperature without affecting product quality.
Caution: To address concerns regarding DENARASE High Salt stability at elevated temperatures during transport, delays in shipping or custom clearances and/or refrigeration problems at the customer locations, the following stability tests were conducted:
Incubation of DENARASE High Salt
I) at 4 °C for 3 months
II) at room temperature for 28 days
III) at 30 °C for 3 days
