TRYPTIC SOY BROTH - BAG is ready to use liquid medium in bag that supports the growth of a wide variety of microorganisms, especially common aerobic and facultatively anaerobic bacteria and fungi, formulated in accordance with ISO 10560:1993. The addition of agar in a concentration 0,1 to 0,2% makes the medium suitable for the growth of obligate anaerobes. The medium is recommended by the United States Pharmacopeia (USP) and the European Pharmacopeia (EP) for counting total anaerobic microorganisms in pharmaceutical products and to test the absence of fungal contamination in pharmaceutical products at 20 to 25 °C.
- Aseptically dispense the required amount of the medium in the containers of choice
- Bring the medium to room temperature or, preferably at 36 ±1 °C
- Open the container just before inoculation. Containers open, closed and not used within a few hours should be discarded
- Inoculate introducing the sample into the container
- Close immediately the container
- Incubate at 36 ±1 °C for 18 to 24 hours
Preparation: Verify that the contents of the bag is homogeneous and clear. TRYPTIC SOY BROTH - BAG can be used as such or divided, under aseptic conditions, in lower aliquots.
Tryptone and soytone are sources of peptides and free amino acids. Glucose is a energy source easy to use by microorganisms. Sodium chloride maintains the osmotic balance of the medium. Dibasic potassium phosphate acts as buffer to pH control.
Caution: The product does not contain hazardous substances in concentrations exceeding the limits set by current legislation and therefore is not classified as dangerous. It is nevertheless recommended to consult the safety data sheet for its correct use. The product is designed for In vitro diagnostic use and must be used only by properly trained operators.
