The E-Z 384 Plant DNA HT Kit enables rapid and high–throughput isolation of genomic DNA from a wide variety of plant tissue samples. Up to 768 fresh, frozen, or dried plant tissue samples rich in polyphenols, polysaccharides or having a lower DNA content can be processed in approximately 1,5 hours when processing two plates in parallel. The system combines the reversible nucleic acid-binding properties of the HiBind® matrix with the speed and versatility of silica plate technology to eliminate polysaccharides, phenolic compounds, and enzyme inhibitors from plant tissue lysates. The purification procedure relies on the well-established properties of the cationic detergent, cetyltrimethyl ammonium bromide (CTAB), in conjunction with a unique binding system to provide high-quality DNA. The system eliminates the need for chloroform extractions traditionally associated with CTAB-based lysis methods. The eluted DNA is inhibitor-free and ready for downstream applications such as genotyping, plant breeding, population level analyses, Next Generation Sequencing, PCR, and more.
- Productivity - Allows processing of up to 768 samples in parallel
- Robust - Reliable results from a variety of sample types
- Safe - No organic extractions
- Performance - High-quality DNA sufficient for most applications
Specifications
Starting Material: Fresh, frozen, or dried plant tissue samples rich in polysaccharides, polyphenols, or those having a low DNA content
Starting Amount: 30 mg fresh/10 mg dry plant sample per well
Elution Volume: 65 µl
Processing Mode: Manual (Centrifugation or Vacuum)
Throughput: 1 to 768 (process two 384-plates in parallel)
Nucleic Acid Binding Technology: 384-well silica filter plate
Processing time: 1,5 h
Downstream
Application(s): Genotyping, plant breeding, population level analyses, next generation sequencing, PCR, and more.
Caution: For Research Use Only. Not for use in diagnostic procedures.
