PIPES ≥99%, white powder for molecular biology

Supplier: MP Biomedicals
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Avg. Ratings: 0.0 out of 5

Synonyms: 2,2'-(Piperazine-1,4-diyl)diethanesulphonic acidPiperazine-1,4-bis(2-ethanesulphonic acid)2,2'-(Piperazine-1,4-diyl)bis(ethanesulphonic) acidPiperazine-N,N'-bis-2-ethanesulphonic acid

ICNA0219483890EA 714 GBP
ICNA0219483890
PIPES ≥99%, white powder for molecular biology
PIPES

PIPES is a zwitterionic N-substituted aminosulfonic acid "Good" buffer.
PIPES is used to buffer glutaraldehyde fixative solutions when fixing plant and/or animal tissue samples. This will help prevent the loss of lipids. It is also used to buffer glutaraldehyde and formaldehyde when fixing fungal zoospores for fluorescence microscopy and electron microscopy. PIPES tends to give optimal results.
Buffers can be made by adding a solution of base to PIPES free acid, titrating to the appropriate pH.


  • Molecular Biology Reagent Grade
  • Presentation: White Powder
  • Effective buffering range: 6.1 - 7.5
  • For sterilization it is recommended to filter sterilize using a 0.2 µm filter.

Formula: C₈H₁₈N₂O₆S₂
MW: 302.37 g/mol
Boiling Pt: 499 °C (1013 hPa)
Melting Pt: > 300 °C (lit.)
Storage Temperature: Ambient
MDL Number: MFCD00006159
CAS Number: 5625-37-6
EINECS: 227-057-6
Merck Index: 12,07633
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Specification Test Results

Identity Test Passes
Purity ≥99%
FTIR Conforms to Standard
UV/VIS: OD260 (0.1M in 0.2M NaOH) ≤0.05
UV/VIS: OD280 (0.1M in 0.2M NaOH) ≤0.05
Solubility (0.1M in 0.2N NaOH) Clear and Complete
pKa (25°C) 6.6-7.0
Moisture (LOD) ≤1.0%

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