PIPES ≥99%, white powder for molecular biology
Supplier: MP BiomedicalsTotal Ratings: 0
Avg. Ratings: 0.0 out of 5
Synonyms:
2,2'-(Piperazine-1,4-diyl)diethanesulphonic acid
, Piperazine-1,4-bis(2-ethanesulphonic acid)
, 2,2'-(Piperazine-1,4-diyl)bis(ethanesulphonic) acid
, Piperazine-N,N'-bis-2-ethanesulphonic acid
PIPES is a zwitterionic N-substituted aminosulfonic acid "Good" buffer.
PIPES is used to buffer glutaraldehyde fixative solutions when fixing plant and/or animal tissue samples. This will help prevent the loss of lipids. It is also used to buffer glutaraldehyde and formaldehyde when fixing fungal zoospores for fluorescence microscopy and electron microscopy. PIPES tends to give optimal results.
Buffers can be made by adding a solution of base to PIPES free acid, titrating to the appropriate pH.
- Molecular Biology Reagent Grade
- Presentation: White Powder
- Effective buffering range: 6.1 - 7.5
- For sterilization it is recommended to filter sterilize using a 0.2 µm filter.
Formula:
C₈H₁₈N₂O₆S₂ MW: 302.37 g/mol Boiling Pt: 499 °C (1013 hPa) Melting Pt: > 300 °C (lit.) Storage Temperature: Ambient |
MDL Number:
MFCD00006159 CAS Number: 5625-37-6 EINECS: 227-057-6 Merck Index: 12,07633 |
Specification Test Results
Identity Test | Passes |
Purity | ≥99% |
FTIR | Conforms to Standard |
UV/VIS: OD260 (0.1M in 0.2M NaOH) | ≤0.05 |
UV/VIS: OD280 (0.1M in 0.2M NaOH) | ≤0.05 |
Solubility (0.1M in 0.2N NaOH) | Clear and Complete |
pKa (25°C) | 6.6-7.0 |
Moisture (LOD) | ≤1.0% |
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