X-Gal (5-bromo-4-chloro-3-indolyl-ß-D-galactopyranoside) ≥98.0%
Supplier: VWR ChemicalsTotal Ratings: 0
Avg. Ratings: 0.0 out of 5
Synonyms:
BCIG
, X-GAL
, (2S,3R,4S,5R,6R)-2-((5-Bromo-4-chloro-1H-indol-3-yl)oxy)-6-(hydroxymethyl)tetrahydro-2H-pyran-3,4,5-triol
X-Gal is a chromogenic substrate for ß-Galactosidase that forms an intense blue precipitate. Used in conjunction with IPTG in a colorimetric assay, X-Gal allows differentiation of recombinants from non recombinants in cloning experiments with vectors containing the lacZ gene. It is also used in microbiology to detect microorganisms which have ß-Galactosidase activity.
Formula:
C₁₄H₁₅BrClNO₆ MW: 408.63 g/mol Melting Pt: 237…239 °C Storage Temperature: Freezer |
MDL Number:
MFCD00005666 CAS Number: 7240-90-6 EINECS: 230-640-8 |
Specification Test Results
Assay | Min. 98.0 % |
Identification | Passes test |
Appearance | White crystalline powder |
Spec.opt. rotation (1 %;DMF/water 50/50) | 60.0 - 64.0 ° |
Water | Max. 1 % |
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