Alcian blue solution used in nuclear staining, for histology, Sigma-Aldrich®

Supplier: Merck
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1.01647.0500EA 147 GBP
1.01647.0500
Alcian blue solution used in nuclear staining, for histology, Sigma-Aldrich®
Alcian blue 8 GX

Used to stain acidic mucosubstances


The combination of PAS staining with Alcian blue allows (at pH 2,5) the additional identification of acidic mucosubstances (glycosaminoglycans). PAS staining is used for paraffin sections and cell smears, and upon use of the ready to use Alcian blue staining solution, nuclei will appear blue, acidic mucosubstances light blue and polysaccharides, as well as neutral mucopolysaccharides, will be stained purple. Additionally, the Alcian blue staining solution (at pH 2,5) allows the identification of acidic mucosubstances without differentiation of carboxyl and sulphate groups, upon which the nuclei are counter-stained with nuclear fast red. As an IVD product and CE registered, it can be used hassle-free in diagnostics and laboratory accreditations.

Formula: C₅₆H₆₈Cl₄CuN₁₆S₄
MW: 1298.86 g/mol
Density: 1.006 g/cm3 (20 °C)
Storage Temperature: Ambient
MDL Number: MFCD00010720
CAS Number: 33864-99-2
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Specification Test Results

Suitability for microscopy passes test
neutral mucopolysaccharides purple
polysaccharides purple
PAS negative acidic mucosubstances light blue
Nuclei blue

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