FLUO 3/AM 1 mM in anhydrous DMSO fluorescent indicator, for calcium determination

Supplier: Biotium
Ratings: (No Reviews)

Total Ratings: 0
Avg. Ratings: 0.0 out of 5


Synonyms: 4-(6-Acetoxymethoxy-2,7-dichloro-3-oxo-9-xanthenyl)-4′-methyl-2,2′(ethylenedioxy)dianiline-N,N,N′,N′-tetraacetic acid tetrakis(acetoxymethyl) ester1-[Amino-5-(2,7-dichloro-6-acetomethoxy-3-oxo-3H-xanthen-9-yl)phenoxy]-2-(2'-amino-5'-methylphenoxy)ethane-N,N,N',N'-tetraacetic acid pentaacetoxy-methyl ester

50015.EA 395 GBP
50015.
FLUO 3/AM 1 mM in anhydrous DMSO fluorescent indicator, for calcium determination
FLUO 3/AM

Membrane-permeant AM ester form of Fluo-3 that can be loaded into cells via incubation. Because of the relatively low water solubility of the AM ester, the mild detergent Pluronic F-127 (catalog no. 59004) is often used as a dispersing agent to facilitate loading. Fluo-3 AM ester itself does not bind Ca2 , but it is readily hydrolyzed to fluo-3 by endogenous esterases once the dye is inside the cells. An important application of Fluo-3 AM ester is its use in high throughput drug screening.


  • Absorption/Emission For Fluo-3: 506 nm/526 nm (low or high [Ca2+] )
  • Extinction Coefficient For Fluo-3: 86,000 M -1 cm -1 (506 nm)
  • Cell permeability: Membrane permeant
  • Orange red solid soluble in DMSO
  • Store -20°C and protect from light, especially when in solution

Formula: C₅₁H₅₀Cl₂N₂O₂₃
MW: 1129.85 g/mol
Storage Temperature: Freezer
CAS Number: 121714-22-5

Order Now

Learn more

About VWR

Avantor is a vertically integrated, global supplier of discovery-to-delivery solutions for...

Learn more About VWR