5(6)-Carboxyfluorescein diacetate ≥95% (by TLC) fluorescent pH indicator

Supplier: Biotium
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Synonyms: 5(6)-Carboxy-di-O-acetylfluorescein5(6)-CFDACFDA

51014.EA 144 GBP
51014.
5(6)-Carboxyfluorescein diacetate ≥95% (by TLC) fluorescent pH indicator
5(6)-Carboxyfluorescein diacetate

5(6)-CFDA (full name: 5-(and-6)-Carboxyfluorescein diacetate) is membrane-permeant and thus can be loaded into cells via incubation.


  • Cell permeability: Membrane permeant
  • Cellular localization: Nucleus & cytoplasm
  • Colors: Green
  • λex\λem 492nm\517nm (pH 9, after hydrolysis)
  • Extinction Coefficient (ε) 78,000 (pH 9, after hydrolysis)
  • Off-white solid soluble in DMSO
  • Store desiccated at 4°C


Once inside cells, CFDA is hydrolyzed by intracellular esterases to carboxyfluorescein (see catalog number 51013).

Formula: C₂₅H₁₆O₉
MW: 460.39 g/mol
MDL Number: MFCD00467494
CAS Number: 124387-19-5

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