EcoRII restriction enzyme recognises ^CCWGG sites and cuts best at 37 °C in O buffer.
- Superior quality-stringent quality control and industry leading manufacturing process
- Convenient colour-coded five buffer system
- Includes universal tango buffer for double-digestions
- BSA premixed in reaction buffers
- Wide selection of restriction endonuclease specificities
Conventional restriction endonucleases are a large collection of high quality restriction enzymes, optimised to work in one of the buffers of the five buffer System. In addition, the universal tango buffer is provided for convenience in double digestions. All of the enzymes exhibit 100% activity in the recommended buffer and reaction conditions. EcoRII restriction enzyme reaction buffers contain premixed BSA, which enhances the stability of many enzymes and binds contaminants that sent in DNA preparations.
At least two copies of EcoRII recognition site are required for efficient cleavage. For cleavage of DNA substrates with only one copy of recognition site MvaI, neoschizomer of EcoRII is recommended. EcoRII may remain associated with the cleaved DNA. This may cause DNA band shifting during electrophoresis. To avoid atypical DNA band patterns, use the 6X DNA Loading Dye and SDS Solution for sample preparation, or heat the digested DNA in the presence of SDS prior to electrophoresis. Assayed using pBR322 DNA (dcm-). EcoRII is blocked by overlapping dcm methylation.
Applications include molecular cloning, restriction site mapping, genotyping, southern blotting, restriction fragment length polymorphism (RFLP) and SNP.
Caution: For research use only. Not for use in diagnostic procedures.
