Tween® 80 (Polysorbate), liquid, Cell Culture Grade
Supplier: MP BiomedicalsTotal Ratings: 0
Avg. Ratings: 0.0 out of 5
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Warning
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Tweens® are a series of nonionic surfactants derived from sorbitan esters. They are soluble or dispersible in water but differ widely in organic and oil solubilities. Used as oil-in-water emulsifiers in pharmaceuticals, cosmetics, cleaning compounds, etc.
Tween® 80 has been widely used in biochemical applications including: solubilizing proteins, isolating nuclei from cells in culture selective protein extraction growing of tubercule bacilli, and emulsifying and dispersing substances in medicinal and food products. It has little or no activity as an anti-bacterial agent. It has been shown to have an adverse effect on the antibacterial effect of methyl paraben and related compounds. Non-ionic detergent used for selective protein extraction and isolation of nuclei from mammalian cell lines.
Soluble/miscible in water to give a clear yellow solution; miscible with alcohol, dioxane, and ethyl acetate; practically insoluble in liquid paraffin and fixed oils (such as mineral oil).
Autoclaving of solutions is not recommended. Sterile filtering is suggested with a 0.22 micron filter. Tween® may need to be warmed to about 40 °C and alternated with portions of hot distilled water while being poured through the filter.
Store at Room Temperature (15-30 °C).
- Cell Culture Reagent Grade
- Concentration: ~75% oleic acid
- Presentation: Liquid
- Critical Micelle Concentration (CMC): 0.012 mM (13-15 mg/L)
Boiling Pt:
100 °C (lit.) Melting Pt: –21 °C Density: 1.08 g/mL Flash Pt: >149 °C Storage Temperature: Ambient |
MDL Number:
MFCD00082107 CAS Number: 9005-65-6 EINECS: 500-019-9 Merck Index: 13,07664 |
Specification Test Results
Identity Test A | Passes |
Identity Test B | Passes |
Identity Test C | Passes |
Water Content | ≤3.0% |
Residue on Ignition | ≤0.25% |
Specific Gravity | 1.06-1.09 |
Viscosity | 300-500cps |
Saponification Value | 45-55 |
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