FLUOR DE LYS® SIRT3 fluorometric drug discovery assay kit

Supplier: Enzo Life Sciences
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FLUOR DE LYS®
ENZOBMLAK5570001EA 805 GBP
ENZOBMLAK5570001 ALEXBML-AK557-0001
FLUOR DE LYS® SIRT3 fluorometric drug discovery assay kit
Assays Cellular Assays
A Fluor-de-Lys® fluorescent assay system. The SIRT3 Fluorescent Activity Assay/Drug Discovery Kit is a complete assay system designed to measure the lysyl deacetylase activity of the recombinant human SIRT3 included in the kit. The kit is ideal for chemical library screening for candidate inhibitors or activators or kinetic assay of the enzyme under varying conditions.

  • Useful for inhibitor screening or characterizing enzyme kinetics
  • Includes optimal substrate selected from a panel of acetylated sites in p53 and histones

The Fluor-de-Lys® SIRT3 assay is based on the Fluor de Lys® Substrate and Fluor de Lys® Developer combination. The assay procedure has two steps. First, the Fluor de Lys® SIRT2 Substrate, which comprises a unique peptide based on amino acids 317-320 of p53 (Gln-Pro-Lys-Lys(Ac)), is incubated with SIRT3. Deacetylation of the substrate sensitizes the substrate so that, in the second step, treatment with the Fluor de Lys®Developer II produces a fluorophore.

Delivery information: Kit includes SIRT3 (Sirtuin 3) (human, recombinant), FLUOR DE LYS® SIRT2 Deacetylase Substrate, FLUOR DE LYS® Developer II Concentrate, NAD (Sirtuin Substrate), Nicotinamide (Sirtuin Inhibitor), Suramin sodium (Sirtuin Inhibitor), FLUOR DE LYS® Deacetylated Standard, Sirtuin Assay Buffer, Half volume microplates, Half volume white microplate.
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