Immobilisation kit, AminoLink™, Pierce™

Supplier: Thermo Fisher Scientific
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Pierce
PIER44890EA 462 GBP
PIER44890
Immobilisation kit, AminoLink™, Pierce™
Protein Purification Protein Purification Kits
AminoLink™ Immobilisation Kit contains AminoLink™ coupling resin and associated reagents sufficient to perform 5 protein immobilisations, with an expected yield of 2 to 20 mg protein per column.

  • Ideal for antibodies and other proteins – immobilise molecules via primary amines (-NH2)
  • Efficient (>85%) coupling over a wide range of pH (4 to 10) and buffer conditions (PBS or other non-amine buffer, with or without organic solvent)
  • Coupling reaction results in stable, leak-resistant secondary amine bond between resin and ligand
  • Better than immobilisation to CNBr-activated agarose as the bond is more stable and uncharged, resulting in less non specific binding in affinity purification procedures
  • Prepared affinity resin is adaptable to column and batch affinity techniques and the resin is reusable for typical applications based on protein binding interactions

The coupling resin is crosslinked 4% beaded agarose that has been activated with aldehyde groups to enable covalent immobilisation of antibodies and other proteins through primary amines.

Accessories information: The AminoLink™ reductant (sodium cyanoborohydride) promotes the formation of stable bonds between aldehyde- and amine-containing molecules, enabling efficient labeling, conjugation, and immobilisation of proteins and other molecules.

Delivery information: Kit includes AminoLink™ columns, 2 ml (5 columns), AminoLink™ coupling buffer pH 7,0 (250 ml), quenching buffer (50 ml), wash buffer (240 ml), sodium cyanoborohydride solution 5 M (0,5 ml), and column accessories.
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