Immobilised jacalin agarose, Pierce™

Supplier: Thermo Fisher Scientific
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Pierce
PIER20395EA 426 GBP
PIER20395
Immobilised jacalin agarose, Pierce™
Protein Purification Protein Purification Reagents
Jacalin is an alpha-D-galactose binding lectin extracted from jack-fruit seeds (Artocarpus integrifolia). This lectin is a glycoprotein (approx. 40 kDa) composed of four identical subunits. Jacalin binds to human IgA1 (also called serum or secretory IgA) with high specificity (i.e., it does not bind IgG or IgM). Thus, Jacalin that has been immobilised on crosslinked beaded agarose is useful for purifying human IgA from serum or colostrum samples.

  • Immobilised jacalin specifically binds human IgA1 (does not bind IgG and does not bind human IgA2 or IgA from other species)
  • Resin is loaded with approx. 4,5 mg Jacalin/ml resin and effectively binds 1 to 3 mg human IgA1/ml resin
  • Agarose resin support is 6% crosslinked beaded agarose, the most popular and versatile resin for protein affinity purification methods

Jacalin Agarose provides for specific affinity purification of human IgA from serum or colostrum or efficient removal of contaminating IgA from IgG samples. Binding of IgA toimmobilized jacalin occurs at physiologic pH and ionic strength, as inphosphate-buffered saline (PBS). Elution of bound IgA occurs withcompetitor ligand (e.g., 0,1 M melibiose or 0,1 M alpha-D-galactose) in PBS. IgD is reported to bind to jacalin.

Caution: For research use only. Not for use in diagnostic procedures.
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