BCIP/NBT alkaline phosphatase substrate
Supplier: Rockland ImmunochemicalsTotal Ratings: 0
Avg. Ratings: 0.0 out of 5
BCIP/NBT Membrane Alkaline Phosphatase Substrate is an artificially manufactured chromogenic substrate for use in sensitive colorimetric assays. Alkaline phosphatase hydrolyzes the BCIP to 5-bromo-4-chloro-3-indole, which is oxidized by oxygen in the atmosphere, yielding a blue dye. In the presence of NBT, an insoluble dark blue precipitate is formed after a reduction reaction instead.
BCIP/NBT Alkaline Phosphatase Membrane Substrate is a useful detection reagent for immunoblotting. The substrate is ready to use. To use: Wash the membrane after incubation with the alkaline phosphatase conjugate secondary reagent. Apply sufficient substrate to cover the membrane. Color (purple-turquoise, varies with pH) will develop within 30 minutes. Wash, dry membrane and image.
Storage Condition: Store container at 4° C prior to opening. Protect BCIP/NBT Membrane Alkaline Phosphatase Substrate from moisture and light.
Specification Test Results
pH | 9.28 +/- 0.1 |
QC Raw Material | PASS |
Absorbance check of final product | PASS |
Performance Data per Immunoblot | PASS |
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