BCIP/NBT alkaline phosphatase substrate

Supplier: Rockland Immunochemicals
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ROCKNBT-100EA 200 GBP
ROCKNBT-100
BCIP/NBT alkaline phosphatase substrate
BCIP/NBT

BCIP/NBT Membrane Alkaline Phosphatase Substrate is an artificially manufactured chromogenic substrate for use in sensitive colorimetric assays. Alkaline phosphatase hydrolyzes the BCIP to 5-bromo-4-chloro-3-indole, which is oxidized by oxygen in the atmosphere, yielding a blue dye. In the presence of NBT, an insoluble dark blue precipitate is formed after a reduction reaction instead.


BCIP/NBT Alkaline Phosphatase Membrane Substrate is a useful detection reagent for immunoblotting. The substrate is ready to use. To use: Wash the membrane after incubation with the alkaline phosphatase conjugate secondary reagent. Apply sufficient substrate to cover the membrane. Color (purple-turquoise, varies with pH) will develop within 30 minutes. Wash, dry membrane and image.


Storage Condition: Store container at 4° C prior to opening. Protect BCIP/NBT Membrane Alkaline Phosphatase Substrate from moisture and light.

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Specification Test Results

pH 9.28 +/- 0.1
QC Raw Material PASS
Absorbance check of final product PASS
Performance Data per Immunoblot PASS

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