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Microsoft Word - GeCKOv2.0 library amplification protocol.docx   Page 1 of 3 rev20140502 Genome-scale CRISPR Knock-Out (GeCKO) v2.0 pooled libraries CRISPR (Clustered Regularly Interspaced Short Palindromic Repeats) is a microbial nuclease system ...

Microsoft Word - GeCKOv2.0 library amplification protocol.docx   Page 1 of 3 rev20140502 Genome-scale CRISPR Knock-Out (GeCKO) v2.0 pooled libraries CRISPR (Clustered Regularly Interspaced Short Palindromic Repeats) is a microbial nuclease system ...

Cell culture ☐ Animal and Human Sera Fetal Bovine Serum (FBS) FBS Alternatives Bovine Serum Albumin (BSA) Other Sera ☐ Classical Media DMEM IMDM F-12 Leibovitz’s L-15 MCDB 131 MEM RPMI ☐ Serum-Free and Specialty Media CHO Insect Serum-Free (Genera...

Cell culture ☐ Animal and Human Sera Bovine Serum Albumin (BSA) Fetal Bovine Serum (FBS) FBS Alternatives Other Sera ☐ Buffered Salt Solutions DPBS EBSS HBSS Other PBS ☐ Cell Counting Automated Manual Viability Reagents ☐ 3D Cell Culture 3D Cell C...

LABORATORY CHECKLIST Genomics ☐ Plasmid Purification Accessories Colony Screening Column Regeneration Endotoxin-Free Plasmid Isolation Rapid Plasmid Isolation Standard High Purity Plasmid Isolation Yeast Plasmid Isolation ☐ Genomic DNA Purificatio...

LABORATORY CHECKLIST Genomics ☐ Plasmid Purification Standard High Purity Plasmid Isolation Rapid Plasmid Isolation Endotoxin-Free Plasmid Isolation Yeast Plasmid Isolation Colony Screening Column Regeneration Accessories ☐ Genomic DNA Purificatio...

PRODUCT CHECKLIST ☐ Cell Biology Antibodies Cell Culture Equipment Cellular Assays and Signal Transduction Extracellular Matrices and Coated Plates Immunoassays Media Classical Mycoplasma Detection and Removal Serum Serum-Free Specialty Supplement...

PRODUCT CHECKLIST ☐ Cell Biology Antibodies Cell Culture Equipment Cellular Assays and Signal Transduction Extracellular Matrices and Coated Plates Immunoassays Media Classical Mycoplasma Detection and Removal Serum Serum-Free Specialty Supplement...

PRODUCT CHECKLIST Equipment ☐ Anaerobic Systems Chambers Indicators Jars ☐ Anesthesia Equipment ☐ Autoclaves/Sterilizers Portable Top-Loading ☐ Blenders ☐ Cell Counters ☐ Centrifuges Benchtop Centrifugal Concentrators Clinical Floor/Ultra Micro/Mi...

Equipment ☐ Anaerobic Systems Chambers Indicators Jars ☐ Anesthesia Equipment ☐ Autoclaves/Sterilizers Portable Top-Loading ☐ Blenders ☐ Cell Counters ☐ Centrifuges Benchtop Centrifugal Concentrators Clinical Floor/Ultra Micro/Mini Refrigerated Ro...

Proteomics PROTEIN EXPRESSION ☐ Protein Expression Mammalian Routine Tightly Controlled Ultra-Pure ☐ Prokaryotic Cells Competent Cells Electroporation Induction Reagents PET Vectors for Gateway Cloning ☐ Transfection Insect Cells Mammalian Cells ☐...

PowerPoint Presentation pGLO™ Transformation and Purification of Green Fluorescent Protein (GFP) Stan Hitomi Coordinator – Math & Science San Ramon Valley Unified School District Danville CA Kirk Brown Lead Instructor Edward Teller Education Cente...

Permeable Supports Quick Reference Ordering Guide Including Transwell® and Falcon® Cell Culture Inserts Quick Reference Ordering Information Uncoated Individual Inserts Transwell® Permeable Supports Polycarbonate (PC) Membrane Membrane Cat. No. De...

Permeable Supports Quick Reference Ordering Guide Including Transwell® and Falcon® Cell Culture Inserts Quick Reference Ordering Information Uncoated Individual Inserts Transwell® Permeable Supports Polycarbonate (PC) Membrane Membrane Cat. No. De...

Permeable Supports Quick Reference Ordering Guide Including Transwell® and Falcon® Cell Culture Inserts Quick Reference Ordering Information Uncoated Individual Inserts Transwell® Permeable Supports Polycarbonate (PC) Membrane Membrane Cat. No. De...

Permeable Supports Quick Reference Ordering Guide Including Transwell® and Falcon® Cell Culture Inserts Quick Reference Ordering Information Uncoated Individual Inserts Transwell® Permeable Supports Polycarbonate (PC) Membrane Membrane Cat. No. De...